上海起發(fā)為Cyandye特約經(jīng)銷商，Cyandye是一家以合理的價(jià)格向處理分子生物學(xué)的科學(xué)家提供化學(xué)服務(wù)的公司。Cyandye的專長(zhǎng)是生物技術(shù)和生命科學(xué)的熒光染料。
貨號(hào) | 品名 | 規(guī)格 | 品牌 |
Lot 10002 | Azidobutyrate NHS Ester | 50 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 100 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 250 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 50 mg | cyandye |
分子式：C 8 H 10 N 4 O 4分子量
：226.19
同義詞：
對(duì)于標(biāo)記0.2微摩爾的氨基修飾的寡核苷酸，在60μL的ACN中溶解2.3mg的疊氮丁酸NHS酯（DMSO或DMF也是可接受的）。將氨基修飾的寡核苷酸溶解在500μL的0.1M碳酸鹽/碳酸氫鹽緩沖液（pH 9）中。加入12μL的Azidobutyrate NHS酯溶液到溶解的寡核苷酸中，并在室溫下反應(yīng)2小時(shí)并脫鹽。
儲(chǔ)存：干燥，-10至-30°C
稀釋劑：無(wú)水ACN，DMSO或DMF溶液
穩(wěn)定性：2-3天
質(zhì)量控制： NMR：95％TLC：純
在這種情況下，在任何合成器上像往常一樣合成寡核苷酸。氨基必須在寡核苷酸的3'或5'端引入。在裂解和脫保護(hù)之后，疊氮基可以作為胺的修飾被引入。例如，碳酸鈉/碳酸氫鈉緩沖液（pH8.75）中的寡核苷酸在室溫下與琥珀酰亞胺基-4-疊氮基丁酸酯在DMSO中孵育。然后使用HPLC純化終的寡核苷酸或用乙醇或丁醇沉淀。
參考文獻(xiàn)：
J. Am?；瘜W(xué)。
使用Click Chemistry
Langmuir，2010，26（11），pp 8666-8672 DOI：10.1021 / ja070273v Template-Directed Oligonucleotide Strand Ligation，Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry Langmuir，2010，26（11），pp 8666-8672 DOI： 10.1021 / la904822f
將脂質(zhì)囊泡共價(jià)連接到液體支持的雙層允許觀察DNA介導(dǎo)的囊泡相互作用
Cyandye 產(chǎn)品列表：
貨號(hào) | 品名 | 規(guī)格 | 品牌 |
Lot 10002 | Azidobutyrate NHS Ester | 50 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 100 mg | cyandye |
Lot 10002 | Azidobutyrate NHS Ester | 250 mg | cyandye |
Lot 10007 | Cy3 carboxylic acid | 5 mg | cyandye |
Lot 10007 | Cy3 carboxylic acid | 100 mg | cyandye |
Lot 10006 | Cy5 carboxylic acid | 5 mg | cyandye |
Lot 10012 | Cy5.5 carboxylic acid | 5 mg | cyandye |
Lot 10012 | Cy5.5 carboxylic acid | 25 mg | cyandye |
Lot 10012 | Cy5.5 carboxylic acid | 50 mg | cyandye |
Lot 10012 | Cy5.5 carboxylic acid | 100 mg | cyandye |
Lot 10013 | diSulfo - Cy3 alkyne | 1 mg | cyandye |
Lot 10013 | diSulfo - Cy3 alkyne | 5 mg | cyandye |
Lot 10013 | diSulfo - Cy3 alkyne | 10 mg | cyandye |
Lot 10013 | diSulfo - Cy3 alkyne | 25 mg | cyandye |
Lot 10022 | diSulfo - Cy3 azide | 1 mg | cyandye |
Lot 10022 | diSulfo - Cy3 azide | 5 mg | cyandye |
Lot 10022 | diSulfo - Cy3 azide | 10 mg | cyandye |
Lot 10022 | diSulfo - Cy3 azide | 25 mg | cyandye |
Lot a0015 | diSulfo - Cy3 carboxylic acid | 5 mg | cyandye |
Lot a0015 | diSulfo - Cy3 carboxylic acid | 25 mg | cyandye |
Lot a0015 | diSulfo - Cy3 carboxylic acid | 50 mg | cyandye |
Lot a0015 | diSulfo - Cy3 carboxylic acid | 100 mg | cyandye |
Lot 10017 | diSulfo - Cy3 NHS Ester | 5 mg | cyandye |
Lot 10017 | diSulfo - Cy3 NHS Ester | 25 mg | cyandye |
Lot 10017 | diSulfo - Cy3 NHS Ester | 50 mg | cyandye |
Lot 10017 | diSulfo - Cy3 NHS Ester | 100 mg | cyandye |
Lot 10016 | diSulfo - Cy5 alkyne | 1 mg | cyandye |
Lot 10016 | diSulfo - Cy5 alkyne | 5 mg | cyandye |
Lot 10016 | diSulfo - Cy5 alkyne | 10 mg | cyandye |
Lot 10016 | diSulfo - Cy5 alkyne | 25 mg | cyandye |
Lot 10023 | diSulfo - Cy5 azide | 1 mg | cyandye |
Lot 10023 | diSulfo - Cy5 azide | 5 mg | cyandye |
Lot 10010 | diSulfo - Cy5 carboxylic acid | 5 mg | cyandye |
Lot 10010 | diSulfo - Cy5 carboxylic acid | 100 mg | cyandye |
Lot 10009 | diSulfo - Cy5 Hydrazide | 5 mg | cyandye |
Lot 10009 | diSulfo - Cy5 Hydrazide | 25 mg | cyandye |
Lot 10009 | diSulfo - Cy5 Hydrazide | 50 mg | cyandye |
Lot 10009 | diSulfo - Cy5 Hydrazide | 100 mg | cyandye |
Lot 10014 | diSulfo - Cy5 NHS Ester | 5 mg | cyandye |
Lot 10014 | diSulfo - Cy5 NHS Ester | 25 mg | cyandye |
Lot 10014 | diSulfo - Cy5 NHS Ester | 50 mg | cyandye |
Lot 10014 | diSulfo - Cy5 NHS Ester | 100 mg | cyandye |
Lot 10027 | diSulfo - Cy7 carboxylic acid | 5 mg | cyandye |
Lot a0021 | diSulfo - Cy7 NHS Ester | 5 mg | cyandye |
Lot a0021 | diSulfo - Cy7 NHS Ester | 25 mg | cyandye |
Lot a0021 | diSulfo - Cy7 NHS Ester | 50 mg | cyandye |
Lot a0021 | diSulfo - Cy7 NHS Ester | 100 mg | cyandye |
Lot 10004 | monoSulfo - Cy3 alkyne | 1 mg | cyandye |
Lot 10004 | monoSulfo - Cy3 alkyne | 5 mg | cyandye |
Lot 10004 | monoSulfo - Cy3 alkyne | 10 mg | cyandye |
Lot 10004 | monoSulfo - Cy3 alkyne | 100 mg | cyandye |
Lot 10011 | monoSulfo - Cy3 azide | 1 mg | cyandye |
Lot 10011 | monoSulfo - Cy3 azide | 5 mg | cyandye |
Lot 10011 | monoSulfo - Cy3 azide | 10 mg | cyandye |
Lot 10011 | monoSulfo - Cy3 azide | 100 mg | cyandye |
Lot 10025 | monoSulfo - Cy3 carboxylic acid | 5 mg | cyandye |
Lot 10025 | monoSulfo - Cy3 carboxylic acid | 25 mg | cyandye |
Lot 10025 | monoSulfo - Cy3 carboxylic acid | 50 mg | cyandye |
Lot 10025 | monoSulfo - Cy3 carboxylic acid | 100 mg | cyandye |
Lot 10008 | monoSulfo - Cy3 NHS Ester | 5 mg | cyandye |
Lot 10008 | monoSulfo - Cy3 NHS Ester | 100 mg | cyandye |
Lot 10020 | monoSulfo - Cy5 azide | 1 mg | cyandye |
Lot 10020 | monoSulfo - Cy5 azide | 5 mg | cyandye |
Lot 10020 | monoSulfo - Cy5 azide | 10 mg | cyandye |
Lot 10020 | monoSulfo - Cy5 azide | 100 mg | cyandye |
Lot 10026 | monoSulfo - Cy5 carboxylic acid | 5 mg | cyandye |
Lot 10026 | monoSulfo - Cy5 carboxylic acid | 25 mg | cyandye |
Lot 10018 | monoSulfo - Cy5 NHS Ester | 5 mg | cyandye |
Lot 10018 | monoSulfo - Cy5 NHS Ester | 25 mg | cyandye |
Lot a0024 | 3-azidopropylamine | 100 mg | cyandye |
Lot d2501 | 2'-Deoxy-5'-acetyl-5-acetoxymethyluridine | 250 mg | cyandye |
Lot a0001 | 2'-Deoxy-5-hydroxymethyluridine | 250 mg | cyandye |
Lot a0001 | 2'-Deoxy-5-hydroxymethyluridine | 500 mg | cyandye |
Lot a0029 | 4-hydrazinobenzene-1,3-disulfonic acid | 500 mg | cyandye |
Lot a0028 | 5-(hydroxymethyl)-1-[(6aR,8R,9aS)-2,2,4,4-tetraisopropyltetrahydro-6H-furo[3,2-f][1,3,5,2,4]trioxadisilocin-8-yl]pyrimidine-2,4(1H,3H)-dione | 50 mg | cyandye |
Lot a0019 | 2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-ol | 500 mg | cyandye |
Lot a0003 | 2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose | 50 mg | cyandye |
Lot a0003 | 2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose | 100 mg | cyandye |
Lot a0003 | 2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose | 250 mg | cyandye |
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